PIAS1 Antikörper (Internal Region)

Produktnummer ABIN7565807

Der Kaninchen Polyklonal Anti-PIAS1-Antikörper wurde für WB, ELISA, DB und FM validiert. Er ist geeignet, PIAS1 in Proben von Human zu detektieren.

Kurzübersicht für PIAS1 Antikörper (Internal Region) (ABIN7565807)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIAS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Produktdetails anti-PIAS1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: PIAS1 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest reactivity to mouse 92.

Produktmerkmale: Synonyms: Rabbit anti-PIAS1 Antibody, Protein Inhibitor Of Activated STAT 1, GBP, ZMIZ3, DDXBP1, DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 1, GU/RH-II, E3 SUMO-protein ligase PIAS1, Gu-binding protein, GBP, AR Interacting Protein

Aufreinigung: Anti-PIAS1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-PIAS1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the internal region of human PIAS1.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-PIAS1 Antibody has been tested for use in ELISA, Western Blotting, and Dot Blotting. HEK293 WCL p/n (W09-000-365) and K-562 WCL p/n (W09-001-GJ7) were used as positive control lysates in western blot. Expect a band at approximately 72 kDa in Western Blots of specific cell lysates and tissues. Specific conditions for reactivity should be optimized by the end user. While not tested, it is likely Anti-PIAS1 antibody will work for immunofluorescence, IHC, and CHIP assays. Western Blot Dilution: 1:1000 ELISA Dilution: 5 μg/mL IF Microscopy Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Andere Bezeichnung: PIAS1

Hintergrund: Background: The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells. Anti-PIAS1 Antibody is useful for researchers interested in epigenetics and cancer research.

Gen-ID: 8554

NCBI Accession: NP_057250

UniProt: O75925

Pathways: JAK-STAT Signalweg, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C