FTH1 Antikörper (HRP)

Produktnummer ABIN7565761

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch FTH1 in WB, IHC, ELISA und FM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für FTH1 Antikörper (HRP) (ABIN7565761)

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FTH1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Produktdetails anti-FTH1 Antikörper (HRP)

Verwendungszweck: Ferritin Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

Produktmerkmale: Synonyms: rabbit anti-Ferritin Antibody HRP Conjugation, peroxidase conjugated rabbit anti-Ferritin Antibody, FTHL6, Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH

Aufreinigung: Anti-Ferritin (Human Spleen) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Ferritin [Human Spleen]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Ferritin (Human Spleen) Peroxidase Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Ferritin [Human Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for Anti-Ferritin (Human Spleen) Antibody. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: User Optimized ELISA Dilution: User Optimized IF Microscopy Dilution: User Optimized Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Andere Bezeichnung: FTH1

Hintergrund: Background: Ferritin is a intracellular protein that directly stores iron and regulates its release, specifically acting as a buffer for iron in humans. Ferritin is composed of 24 subunits and comes in two types, H and L. The H subunit detoxifies iron while the L subunit deals with long term storage of iron. Depending on the concentration of ferritin in the blood, different disorders and diseases could be present in an individual. Anti-Ferritin (Human Spleen) Antibody is ideal for investigators in Cancer, Immunology, and Cardiovascular Research.

Gen-ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis