GLB1 Antikörper (FITC)

Produktnummer ABIN7565749

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GLB1 in WB, DB und FM. Er zeigt eine Reaktivität gegenüber E. coli.

Kurzübersicht für GLB1 Antikörper (FITC) (ABIN7565749)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLB1 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Dot Blot (DB), Fluorescence Microscopy (FM)

Produktdetails anti-GLB1 Antikörper (FITC)

Verwendungszweck: Beta Galactosidase Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Beta Galactosidase (E.

Produktmerkmale: Synonyms: rabbit anti-Beta Galactosidase Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal FITC conjugated antibody, ß-Gal Fluorescein, Anti-ß-Gal Fluorescein Antibody

Aufreinigung: Anti-Beta Galactosidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Beta Galactosidase (E. coli)

Immunogen Type: Native Protein

Labeling Ratio: 2.3

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Beta Galactosidase Fluorescein Conjugated Antibody has been tested by dot bot and western blot and is designed for fluorescent western blotting and immunofluorescence. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Western Blot Dilution: User Optimized IF Microscopy Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Andere Bezeichnung: Beta Galactosidase

Hintergrund: Background: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.

NCBI Accession: NP_414878

UniProt: P00722

Pathways: Glycosaminoglycan Metabolic Process