Rekombinanter TCR gamma/delta Antikörper

Produktnummer ABIN7543458

Der Maus Monoklonal anti-TCR gamma/delta Antikörper wird verwendet zum Nachweis von TCR gamma/delta in Proben von Human. Er wurde validiert für FACS und Func.

Kurzübersicht für Rekombinanter TCR gamma/delta Antikörper (ABIN7543458)

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TCR gamma/delta Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Functional Studies (Func)

Klon: G5-4

Produktdetails anti-TCR gamma/delta Antikörper

Verwendungszweck: Anti-Gamma Delta TCR [G5-4], Mouse IgG2a, kappa

Spezifität: This antibody is specific for the human TCR delta and gamma chains. The T-cell receptor (TCR) is a molecule found on the surface of T cells, or T lymphocytes, that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. On activation through antigen presenting cells (APCs), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. TCR part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant gamma9delta2 (OT3)-Fc fusion protein as an immunogen.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The binding of this antibody to γδ T cells was determined using flow cytometry. This antibody can compete with commercially available anti-γδ TCR antibody IMMU510 for binding to gamma-delta T cells. It is proposed that, since there is some competition between the two antibodies, G5-4 binds a similar or identical epitope to IMMU510. In an in vitro proliferation assay, this antibody was reported to positively stimulate the proliferation of γδT cells. The scFv version of this antibody was also reported to stimulate the secretion of cytokines like IFN-γ and TGF-α. G5-4ScFv-amplified γδT cells can effectively kill daudi cells, and the killing efficiency is greater than 85 % when the effect-to-target ratio is 5:1 (CN103130894).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu TCR gamma/delta

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Andere Bezeichnung: Gamma Delta TCR

Hintergrund: TCR γδ, γδ TCR, TCR GD, GD TCR, TCRg/d, T-cell surface glycoprotein CD3 delta chain, CD3D, T3D, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 gamma chain, CD3G, T-cell receptor T3 gamma chain, CD3g, T3G

UniProt: P04234, P09693