Rekombinanter Iba1 Antikörper

Produktnummer ABIN7543455

Der Ziege Monoklonal anti-Iba1 Antikörper wird verwendet zum Nachweis von Iba1 in Proben von Human. Er wurde validiert für WB, ELISA, IF und FACS.

Kurzübersicht für Rekombinanter Iba1 Antikörper (ABIN7543455)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Ziege

Klonalität: Monoklonal

Konjugat: Dieser Iba1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: GT1-mAb1

Produktdetails anti-Iba1 Antikörper

Verwendungszweck: Anti-AIF1 [GT1-mAb1], Goat IgG, lambda

Spezifität: This antibody is specific to < a href="https://everestbiotech.com/product/ebp05419-immunizing-peptide/" target="_blank">EBP05419, an immunizing peptide with the sequence C-TGPPAKKAISELP. This peptide was used in the production of the < a href="https://everestbiotech.com/product/goat-anti-aif1-iba1-isoform-1-and-3-antibody/" target="_blank">polyclonal Goat Anti-AIF1 antibody from which this antibody is derived.

Produktmerkmale:

Original Species of Ab: Goat

Original Format of Ab: IgG

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP.

Isotyp: IgG lambda

Anwendungsinformationen

Applikationshinweise: This antibody (GT1-mAb1) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb1 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb1 was found to bind the target peptide in a non-competitive manner with GT1-mAb2 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb1 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb1 was compatible with immunofluorescence (IF), flow cytometry (FC), and Western blot (WB), with an FC signal observedly more distinct than that of GT1-mAb2.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: AIF1

Hintergrund: AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, Daintain, DADB-70P7.8, allograft inflammatory factor-1 splice variant Hara-1, IRT1, protein G1

UniProt: P55008

Pathways: Smooth Muscle Cell Migration