Rekombinanter CD24 Antikörper

Produktnummer ABIN7543435

Dieses Anti-CD24-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von CD24 in FACS, WB, IHC und IF. Geeignet für Maus.

Kurzübersicht für Rekombinanter CD24 Antikörper (ABIN7543435)

Target: CD24 (CD24 Molecule (CD24))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD24 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: M1-69

Produktdetails anti-CD24 Antikörper

Verwendungszweck: Anti-CD24 [M1/69], Rabbit IgG, kappa

Spezifität: This antibody binds mouse CD24 also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a variably glycosylated GPI-anchored membrane protein expressed on the surface lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. This protein plays an important role in differentiation of different cell types and its expression varies during lymphocyte differentiation which makes it a good marker for delineating various lymphocyte developmental stages. It is also expressed in erythroleukemia, small cell lung carcinoma and multiple sclerosis.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing DA rats with B10 mouse splenic T cells and concanavalin A-activated splenocytes.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody was capable of recognizing CD24 antigen from microsomal membrane preparation of different mouse cells through western blot (PMID: 1696896). This antibody is recommended for immunophenotypic analysis of CD24 expressed on mouse B and T cells using flow cytometry (PMID: 17702899, 20133615, 20203098, 33111080). This antibody (dilution 1:100) was also used in the immunohistochemical analysis of CD24 expression in mouse thyroid cells using 5-μm zinc-fixed, paraffin-embedded thyroid sections (PMID: 18801910). This antibody was also reported to be used in the flow cytometric and immunohistochemical analysis of murine CD24 expressed on the surface of normal human breast epithelial cells early after their infection with a retroviral vector (PMID: 9232274). This antibody was also used in the immunofluorescence staining of murine spinal cord sections (PMID: 17475850).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD24

Target: CD24 (CD24 Molecule (CD24))

Andere Bezeichnung: CD24

Hintergrund: Heat stable antigen, Signal transducer CD24, Lymphocyte antigen 52, Ly-52, M1/69-J11D heat stable antigen, HSA, Nectadrin, R13-Ag, X62 heat stable antigen

UniProt: P24807

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation