Rekombinanter Mesothelin Antikörper (Extracellular Domain)

Produktnummer ABIN7543372

Dieses Anti-Mesothelin-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von Mesothelin in ELISA, WB, IHC, FACS, IP und SPR. Geeignet für Human.

Kurzübersicht für Rekombinanter Mesothelin Antikörper (Extracellular Domain) (ABIN7543372)

Target: Mesothelin (MSLN)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Mesothelin Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Surface Plasmon Resonance (SPR)

Klon: MN-1

Produktdetails anti-Mesothelin Antikörper

Bindungsspezifität: Extracellular Domain

Verwendungszweck: Anti-Mesothelin [MN-1], Rabbit IgG, kappa

Spezifität: This antibody can recognize and bind human mesothelin. This antibody binds a different antibody on the human mesothelin than another antibody called MB. Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein highly expressed in several types of malignant tumors sometimes in association with increased tumor aggressiveness and poor clinical outcome. The membrane-anchored forms of this molecule may play a role in cellular adhesion.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated in mesothelin-deficient mice by immunization with plasmid cDNA corresponding to the mesothelin extracellular domain followed by a single boost with a recombinant human mesothelin-rabbit IgG Fc fusion protein.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The binding characterization of this antibody was done using ELISA and SPR. The binding affinity of the original IgG2a antibody for mesothelin-Fc protein was determined by BIACore and reported to be Kd = 1.0 nM. This antibody can recognize membrane bound mesothelin on the surface of mesothelin positive H226, A1847, Hela and Panc3.014 cells (mesothelin-positive) in a flow cytometry. This antibody can also bind denatured human mesothelin in a western blot assay. This antibody was also used in the immunohistochemical staining of cancerous tissue like ovarian adenocarcinoma and normal tissues like peritoneal mesothelium on uterus, pleural mesothelium on lung and fallopian tube luminal fluid (PMID: 16115924). This antibody was also used in an immunohistochemical analysis of 1562 tumors for mesothelin expression. It was reported that mesothelin was highly expressed in ovarian serous carcinoma, pancreatic adenocarcinoma and malignant pleural mesothelioma (PMID: 28460459). This antibody was also used in the immunoprecipitation of mesothelin from cell lysates (PMID: 23694968).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Mesothelin

Target: Mesothelin (MSLN)

Andere Bezeichnung: Mesothelin

Hintergrund: MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, MN

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process