Rekombinanter FAP Antikörper

Produktnummer ABIN7543358

Dieses Kaninchen Chimeric-Antikörper erkennt spezifisch FAP in WB, IP, FACS, in vitro und in vivo. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Rekombinanter FAP Antikörper (ABIN7543358)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser FAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), In vitro Assay (in vitro), In vivo Studies (in vivo)

Klon: 73-3

Produktdetails anti-FAP Antikörper

Verwendungszweck: Anti-FAP [73.3], Rabbit IgG, kappa

Spezifität: The antibody is specific for fibroblast activation protein (FAP). FAP is a membrane protease that is highly expressed by cancer-associated fibroblasts (CAFs).

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3ζ and 4-1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35-50 % following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014, PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019, PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016, PMID: 26663085).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Andere Bezeichnung: FAP

Hintergrund: Fibroblast activation protein, Fibroblast activation protein-α, Prolyl endopeptidase FAP, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, FAPalpha1, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme,Serine integral membrane protease, SIMP, Surface-expressed protease, Seprase

UniProt: P97321

Pathways: Tube Formation