Rekombinanter Glypican 3 Antikörper (Extracellular Domain)

Produktnummer ABIN7543338

Der Kaninchen Chimeric Anti-Glypican 3-Antikörper wurde für FACS, WB, IHC und ELISA validiert. Er ist geeignet, Glypican 3 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Glypican 3 Antikörper (Extracellular Domain) (ABIN7543338)

Target: Glypican 3 (GPC3)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Glypican 3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Klon: 7H1

Produktdetails anti-Glypican 3 Antikörper

Bindungsspezifität: Extracellular Domain

Verwendungszweck: Anti-Glypican 3 [7H1], Rabbit IgG, kappa

Spezifität: This antibody binds an epitope between amino acids 25 to 137 of the human glypican 3 (GPC3). This antibody cross reacts with cynomolgus monkey, rat and mouse GPC3. Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC).

Kreuzreaktivität: Cynomolgus, Maus, Ratte

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with a recombinant human GPC3 extracellular domain (ECD, amino acids of 1-547) fused to a C-terminal Flag (RADYKDDDDK).

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody can bind human glypican 3 expressed on the surface of 293S cells, HepG2 X1 cells and JHH7 cells in a flow cytometric assay. The binding characterization of this antibody to huGPC3, cynoGPC3, and HepG2 cells was done using ELISA and immunohistochemistry. This antibody strongly reacts with hepatic cancer tissue microarray, JHH cells, HepG2 cells, and cells stably transfected with GPC3 in immunohistochemistry. This antibody also detects human, cynomolgus monkey, rat, and mouse GPC3 by western blot. This antibody was also used in the generation of an antibody drug conjugate with maleimide acetal PNU-159682 or monomethyl disulfide N10-linked PBD. The ADCC activity of the antibody drug conjugate was investigated using a human HepG2 X1 and JHH7 xenograft model and substantial tumor growth inhibition was achieved with 7H1-disulfide-PBD (US9926377).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Glypican 3

Target: Glypican 3 (GPC3)

Andere Bezeichnung: Glypican 3

Hintergrund: GPC3, Glypican-3, GPC-3, GTR2-2, MXR7, OCI5, DGSX, Intestinal protein OCI-5

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process