Rekombinanter Keratin 5/6 Antikörper (AA 488-590)

Produktnummer ABIN7543311

Der Kaninchen Chimeric anti-Keratin 5/6 Antikörper wird verwendet zum Nachweis von Keratin 5/6 in Proben von Human. Er wurde validiert für IHC und ELISA.

Kurzübersicht für Rekombinanter Keratin 5/6 Antikörper (AA 488-590) (ABIN7543311)

Target: Keratin 5/6 (KRT5/6)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Keratin 5/6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA

Klon: AbM58019-7H4

Produktdetails anti-Keratin 5/6 Antikörper

Bindungsspezifität: AA 488-590

Verwendungszweck: Anti-Cytokeratin 5/6 [AbM58019-7H4], Rabbit IgG, kappa

Spezifität: This antibody is capable of binding human cytokeratin 5 and human cytokeratin 6. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 5/6 is often expressed in a basal cell layer of normal mammary ducts. Studies have also shown that CK5/6 can be used as a characteristic marker of basal-like breast cancer.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino acids 488-590 of cytokeratin 5 in equal volume of Freund's incomplete adjuvant.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The binding characterization of this antibody was done using ELISA. The original IgG2b antibody binds cytokeratin 5/6 with a high affinity constant of 3.84 x 10^(9) M^(-1). This antibody was also used in the immunohistochemical analysis of esophagus and esophageal squamous cell carcinoma. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody D5/16B4 (CN113845592).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Keratin 5/6

Target: Keratin 5/6 (KRT5/6)

Andere Bezeichnung: Cytokeratin 5/6

Hintergrund: CK5/6, CK5, CK-5, K5 KRT5, Keratin, type II cytoskeletal 5, cytokeratin-5, 58 kDa cytokeratin, Type-II keratin Kb5, CGMCC NO 21982, D5/16B4

UniProt: P13647