Rekombinanter Liver Arginase Antikörper

Produktnummer ABIN7543254

Der Kaninchen Chimeric Anti-Liver Arginase-Antikörper wurde für cryo-EM und Inh validiert. Er ist geeignet, Liver Arginase in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Liver Arginase Antikörper (ABIN7543254)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Liver Arginase Antikörper ist unkonjugiert

Applikation: Cryogenic electron microscopy (cryo-EM), Inhibition (Inh)

Klon: MAb1

Produktdetails anti-Liver Arginase Antikörper

Verwendungszweck: Anti-Arginase 1 [mAb1], Rabbit IgG, kappa

Spezifität: The antibody sterically blocks the arginase 1 active site near the exterior surface of the enzyme. Approximately 80 % of all complexes identified were composed of two arginase 1 trimers and three antibodies, forming a 2:3 complex, while the remaining 20 % of complexes were composed of two arginase 1 trimers and two antibodies, however, the epitope and paratope interactions between each antibody and arginase 1 monomers are identical. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The antibody is a human antibody identified via yeast display technologies and isolated after panning against arginase 1.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of human arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki for the antibody against human Arg1 was 3.3 ± 0.3 nM (Palte et al., 2021, PMID: 34326456).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Andere Bezeichnung: Arginase 1

Hintergrund: ARG1, Arginase-1, Liver-type arginase, Type I arginase

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin