Rekombinanter CEA Antikörper

Produktnummer ABIN7543224

Der Kaninchen Chimeric Anti-CEA-Antikörper wurde für IHC, FACS, ELISA, RIA und ISG validiert. Er ist geeignet, CEA in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter CEA Antikörper (ABIN7543224)

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CEA Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Radioimmunoassay (RIA), Immunoscintigraphy (ISG)

Klon: T84-66

Produktdetails anti-CEA Antikörper

Verwendungszweck: Anti-CEA [T84.66], Rabbit IgG, kappa

Spezifität: This antibody binds the A3 domain of the human carcinoembryonic antigen.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing female BALB/c mice subcutaneously with soluble CEA or with the HC84S cell line.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The binding affinity of the original mouse antibody to carcinoembryonic antigen was determined using a competitive radioimmunoassay (PMID: 6187848). 111In (Indacea) labelled version of this antibody was used to image tumors in patients with colorectal cancer (PMID: 3779658). An engineered antibody fragment (VL-VH-CH3, or "minibody") generated using this antibody having bivalent binding to carcinoembryonic antigen showed high level targeting of xenografts (PMID: 8674062). A radiolabeled chimeric version of this antibody was used to target breast cancer cells expressing high levels of CEA and it was found that chimeric 86.66 resulted in good tumor localization as well as significant tumor growth inhibition. This antibody was also used to detect CEA expression in Human breast carcinoma cell line, MCF 7, using flow cytometry and immunohistochemistry (PMID: 21597719). The characterization of the chimeric version of this antibody generated by transient expression in tobacco leaves was done using ELISA (PMID: 10500141). A phase 1 clinical radioimmunotherapy trial studying 22 patients with metastatic CEA-producing malignancies involving 90Yttrium-labeled chimeric T86.44 revealed that the antibody was well tolerated, with reversible thrombocytopenia and leukopenia being dose limiting (PMID: 11051230). This antibody was used in the immunohistochemical staining of paraffin sections of primary tumours from 252 consecutive patients with breast carcinomas (PMID: 9635845).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Andere Bezeichnung: CEA

Hintergrund: Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CEACAM5, Meconium antigen 100, CD66e

UniProt: P06731