Rekombinanter Disialoganglioside 2 (GD2) Antikörper

Produktnummer ABIN7543163

Dieser Kaninchen Chimeric Antikörper detektiert spezifisch Disialoganglioside 2 in FACS und ELISA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter Disialoganglioside 2 (GD2) Antikörper (ABIN7543163)

Target: Disialoganglioside 2 (GD2)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA

Klon: 14G2a

Produktdetails

Verwendungszweck: Anti-Disialoganglioside 2 [14G2a], Rabbit IgG, kappa

Spezifität:

This antibody binds the GD2 antigen and GD2 expressing neuroblastoma cell lines with an affinity similar to the parent antibody.

Application Notes original Clone: This antibody is a IgG2a isotype switch variant of the murine parent antibody 14.18. This antibody can direct efficient complement-dependent cytotoxicity against neuroblastoma tumor cells and melanoma cell lines in vitro in the presence of human complement (PMID: 2720646). 14G2a was coupled to the ribosome-inactivating plant toxin gelonin with the heterobifunctional cross-linking reagentN-succinimidyl-3(2-pyridyldithio)propionate. The immunoreactivity and specificity of 14G2a-gelonin immunotoxin determined by ELISA. Assays for complement-dependent cytotoxicity (CDC) and antibody-dependent cellular cytotoxicity (ADCC) revealed that these functional properties of the native 14G2a antibody were also preserved in the 14G2a-gelonin immunotoxin (PMID: 1756537). An immunotoxin prepared by linked 14G2a via the bivalent linker N-succinimidyloxycarbonyl-amethyl-a-(2-piridyldithio)toluene to deglycosylated ricin A chain was shown to inhibit the protein synthesis of neuroblastoma cell lines IMRS and NMB (Gottstein et al., 1994). Treatment with 14G2a mAb (1-4 mg/kg cumulative dose given as five i.v. daily administrations) dramatically reduced the metastatic spread of NB cells and prolonged the long-term survival of treated mice in a dose-dependent manner (PMID: 12880983). This antibody is also reported to be used in the study of interactions between peptides mimicking the GD2 ganglioside using flow ctyometry and ELISA (PMID: 21455557).

Produktmerkmale: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Aufreinigung: Protein A affinity purified

Immunogen: The original murine parent antibody was generated against human neuroblastoma cell line LAN-1. This IgG2a isotype version was then generated by class switching of the murine parent antibody 14.18 (IgG3).

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Antigendetails

Target: Disialoganglioside 2 (GD2)

Andere Bezeichnung: Disialoganglioside 2

Hintergrund: GD2