Rekombinanter Mesothelin Antikörper

Produktnummer ABIN7543155

Der Maus Monoklonal Anti-Mesothelin-Antikörper wurde für ELISA, WB, IHC, IF, SPR und RIA validiert. Er ist geeignet, Mesothelin in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Mesothelin Antikörper (ABIN7543155)

Target: Mesothelin (MSLN)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Mesothelin Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Surface Plasmon Resonance (SPR), Radioimmunoassay (RIA)

Klon: K1

Produktdetails anti-Mesothelin Antikörper

Verwendungszweck: Anti-Mesothelin [K1], Mouse IgG1, kappa

Spezifität: This antibody can bind the human mesothelium, expressed on many cancer cells like ovarian carcinoma.

Kreuzreaktivität: Cynomolgus

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunization of mice with periodate-treated human ovarian cancinoma (OVCAR-3) cells. These mice had previously been made tolerant to normal human kidney membranes.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This antibody was used for the study and characterization of CAK1 antigen present on ovarian cancers and normal mesothelium. The initial screening of the mesothelin reactive clones to live OVCAR-3 cells was done using immunofluorescence. Secondary screening was done using immunoperoxidase histochemistry on cryostat sections of normal human tissues and human tumors. Quantitative ELISA confirmed that K1 and OC125 antibodies bind to different epitopes on the OVCAR-3 cells (PMID: 1735605). This antibody can detect mesothelin in a western blot assay. Competition RIA was used to detect the presence of CAK1 antigen in the supernatent of protease or phosphatidylinositol-phospholipase C treated cells. An immunotoxin composed of K1 antibody and native Pseudomonas exotoxin was found to be selectively cytotoxic to cells expressing CAK1 antigen (PMID: 1727378). The affinity of K1 antibody to mesothelin was Kd= 8 nM and determined using surface plasmon resonance (BIAcore assay) (PMID: 9178820).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Mesothelin

Target: Mesothelin (MSLN)

Andere Bezeichnung: Mesothelin

Hintergrund: MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process