Rekombinanter IL2 Receptor beta Antikörper

Produktnummer ABIN7543149

Der Maus Monoklonal Anti-IL2 Receptor beta-Antikörper wurde für FACS, ELISA, IP, BR und InhA validiert. Er ist geeignet, IL2 Receptor beta in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter IL2 Receptor beta Antikörper (ABIN7543149)

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL2 Receptor beta Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Klon: Mik-beta 1

Produktdetails anti-IL2 Receptor beta Antikörper

Verwendungszweck: Anti-IL-2R Beta chain [Mik-beta 1], Mouse IgG2a, kappa

Spezifität: This antibody recognizes and binds the beta chain of the IL-2 receptor and completely inhibits the IL-2 binding to the beta chain. The beta subunit is involved in receptor mediated endocytosis and transduction of the mitogenic signals of IL2.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BLAB/c mice with YTS cells, a human NK-like cell line that highly expresses the human IL-2R beta chain as immunogen.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Mik-beta 1 inhibited binding of iodine labelled IL-2 to YTS cells in a dose dependent manner. Mik-beta 1 also successfully immunoprecipitated beta chain of the IL-2R from radio iodinated YTS extract. Biotinylated Mik-beta 1 also efficiently competes with IL-2 for binding to solubilized beta chain. Flow cytometry was used to study the cellular disctribution of beta chain, as determined by mik-beta 1 staining. This antibody completely inhibited IL-2 dependent T cell growth in presence of anti-Tac. Two-color flow cytometric analysis using anti-beta chain mAbs clearly showed that approximately 10 % of peripheral blood lymphocytes manifest the beta chain and that this population is mainly composed of Leu-19+ NK cells (PMID: 2467293). Mik beta 1 antibody acts synergistically with humanized anti-Tac to prevent IL-2-induced proliferation of activated T cells (PMID: 8335891). A recombinant single chain immunotoxin of Mik-beta 1 and Pseudomonas derived exotoxin PE40 was selectively cytotoxic for cells expressing high levels of IL-2R beta (p75) subunit (PMID: 1401913).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Andere Bezeichnung: IL-2R Beta chain

Hintergrund: CD122, IL-2R, IL2Rβ, IL-2RB, p70-75, p75, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, Mik-β1

UniProt: P14784

Pathways: JAK-STAT Signalweg, Growth Factor Binding