Rekombinanter N-Cadherin Antikörper (Extracellular Domain)

Produktnummer ABIN7543136

Dieser Kaninchen Chimeric Antikörper detektiert spezifisch N-Cadherin in WB, IHC, FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human, Kaninchen und Hamster.

Kurzübersicht für Rekombinanter N-Cadherin Antikörper (Extracellular Domain) (ABIN7543136)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Kaninchen, Hamster

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser N-Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 8C11

Produktdetails anti-N-Cadherin Antikörper

Bindungsspezifität: Extracellular Domain

Verwendungszweck: Anti-N-cadherin [8C11], Rabbit IgG, kappa

Spezifität:

This antibody recognizes human, rabbit and hamster extracellular domain of N-cadherin. It does not cross react with this protein in mouse, rat cow and pig. N-cadherin is a calcium-dependent cell adhesion protein.

Application Notes original Clone: 8C11 antibody inhibits cells' motility, as it blocks extracellular domain of N-cadherin which is responsible for this function (Kim et al., 2000). This antibody has also been widely used in the detection and analysis of N-cadherin. For instance, it was utilized to identify this protein in A549 cells via immunofluorescence (Shintani et al., 2011). Another group used successfully 8C11 to analyze the levels of N-cadherin expression via Western blot (Chen et al., 2015). Finally, this antibody was used to label N-cadherin in tumor tissues of a metastasis model via immunohistochemistry (Li et al., 2019).

Kreuzreaktivität: Hamster, Kaninchen

Produktmerkmale: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with an extracellular domain of the recombinant human N-cadherin.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: N-cadherin

Hintergrund: CD325, Cadherin-2, CDw325, Neural cadherin, n-cadherin, ncadherin

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane