Rekombinanter CTNNA1 Antikörper (AA 135-226)

Produktnummer ABIN7543128

Der Kaninchen Chimeric anti-CTNNA1 Antikörper wird verwendet zum Nachweis von CTNNA1 in Proben von Human und Maus. Er wurde validiert für WB, IF und IP.

Kurzübersicht für Rekombinanter CTNNA1 Antikörper (AA 135-226) (ABIN7543128)

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CTNNA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 1G5

Produktdetails anti-CTNNA1 Antikörper

Bindungsspezifität: AA 135-226

Verwendungszweck: Anti-alpha catenin [1G5], Rabbit IgG, kappa

Spezifität:

This antibody recognizes alpha catenin and binds to the domain between amino acids 135-226. α-catenin associates with the cytoplasmic domain of a variety of plasma membrane spanning cadherins that mediate cell-cell adhesion. The association of catenins to cadherins produces a complex that is linked to the actin filament network, which seems to be of primary importance for their cell-adhesion properties.

Application Notes original Clone: This antibody is recommended for the detection and analysis of alpha-catenin via various methods. For instance, it was used to identify alpha-catenin in MDA468 cells via immunofluorescence (Bajpai et al., 2009). It was also repeatedly demonstrated that 1G5 specifically detects alpha-catenin on Western blots (Johnson et al., 1993, Stadler et al., 2018).

Kreuzreaktivität: Maus

Produktmerkmale: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunizing Balb/c mice with a 90 kDa MBP/alpha catenin fusion protein purified on an amylose column.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Andere Bezeichnung: alpha catenin

Hintergrund: Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, α-catenin, α catenin, α(E)-catenin

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location