Rekombinanter Podoplanin Antikörper (AA 38-51)

Produktnummer ABIN7543076

Dieses Ziege Chimeric-Antikörper erkennt spezifisch Podoplanin in WB, IHC, ELISA, FACS und BR. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Rekombinanter Podoplanin Antikörper (AA 38-51) (ABIN7543076)

Target: Podoplanin (PDPN)

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Ziege

Klonalität: Chimeric

Konjugat: Dieser Podoplanin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Blocking Reagent (BR)

Klon: PMab-1

Produktdetails anti-Podoplanin Antikörper

Bindungsspezifität: AA 38-51

Verwendungszweck: Anti-Podoplanin (MAP tag) [PMab-1], Goat IgG, kappa

Spezifität: This antibody is specific for amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin (PDPN). This antibody, therefore, possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), which is derived from the PLAG domain of murine podoplanin. PDPN is a type I transmembrane protein that interacts with the platelet receptor C-type lectin-like receptor-2 (CLEC-2). PDPN is expressed by a wide variety of cells, including podocytes, lymphatic endothelial cells and type I alveolar cells. PDPN is also upregulated on certain cancer cells, where it mediates tumour-induced platelet aggregation by binding to CLEC-2 on platelets. Chihara (2018) associated Podoplanin with a co-inhibitory receptor in T-cell activation along with PD-1, TIM-3, LAG-3 and TIGIT.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody binds to the PLAG domain of murine PDPN and is, therefore, able to block its interaction with C-type lectin-like receptor-2 (CLEC-2) (Yamada, 2017). This has a number of potential therapeutic applications, including the suppression of lymphangiogenesis and inflammatory macrophage infiltration at the site of wound healing in mouse corneal suture and ear section models (Maruyama et al, 2014), as well as suppression of transplant rejection. This antibody possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), and can, therefore, be used to detect and purify tagged proteins in a range of assay formats (Fujii et al, 2016). PMab-1 performs well in immunohistochemical analysis, and has been used to stain the mouse melanoma B16-F10 and mouse podoplanin-expressing CHO transfectants (Kaji et al, 2012). The use of this antibody in immunostaining of somatic tissues, including mouse lung lymphatic vessels, kidney glomeruli, pulmonary alveoli, pulmonary pleura and salivary gland myoepithelial cells, has been successfully performed on both paraffin-embedded sections and frozen sections (Kaji et al, 2012). The specificity of this antibody has been confirmed in Western blot analysis (Kaji et al, 2012), while Western blot analysis has also revealed that activation of LPS-induced inflammatory pathways, including the MAPK and NF-κB pathways, is affected by PMab-1 (Maruyama et al, 2014). The mouse-rat chimeric antibody mPMab-1 is derived from PMab-1 (Yamada et al, 2017).

Kommentare:

This chimeric goat antibody was made using the variable domain sequences of the original rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Podoplanin

Target: Podoplanin (PDPN)

Andere Bezeichnung: Podoplanin

Hintergrund: PDPN, Aggrus, Glycoprotein 38, Gp38, OTS-8, Ots8, PA2.26 antigen, T1-alpha, T1A, Transmembrane glycoprotein E11

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport