Podoplanin Antikörper (AA 24-126)

Produktnummer ABIN6939085

Zitiert in 1 Publikation. Der Maus Monoklonal anti-Podoplanin Antikörper (Klon PDPN-1433) (ABIN6939085) detektiert spezifisch Podoplanin in IHC, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Podoplanin Antikörper (AA 24-126) (ABIN6939085)

Target: Podoplanin (PDPN)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Podoplanin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Klon: PDPN-1433

Produktdetails anti-Podoplanin Antikörper

Bindungsspezifität: AA 24-126

Verwendungszweck: Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Antibody

Spezifität: It recognizes a muco-protein of 38-43 kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Kreuzreaktivität (Details): Human,

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human Podoplanin (PDPN) protein fragment (around aa 24-126) (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Cervical or Lung Carcinoma.

Known Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Referenzen für anti-Podoplanin Antikörper (ABIN6939085)

Wirsig, Bürger, Fleischhauer, Preuß, Bernhardt: "Vascularized in vitro bone model as 3D quadruple culture with primary human osteoblasts, osteocytes, osteoclasts and endothelial cells." in: Materials today. Bio, Vol. 34, pp. 102154, (2025) (PubMed).

Details zu Podoplanin

Target: Podoplanin (PDPN)

Andere Bezeichnung: PDPN

Hintergrund:

Aggrus, Glycoprotein 36 KD, Glycoprotein 36, gp36, GP38, GP40, HT1A1, hT1alpha1, hT1alpha2, Lung type I cell membrane associated glycoprotein, Lung type I cell membrane associated glycoprotein T1A 2, OTS8, PA2.26, Pdpn, Podoplanin, PSEC0003, PSEC0025, T1-alpha, T1A, TI1A, TIA2

HeLa cells. Cervical or Lung Carcinoma.

Molekulargewicht: 38-43kDa

Gen-ID: 10630, 468675

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport