Rekombinanter CD53 Antikörper

Produktnummer ABIN7543047

Der Kaninchen Chimeric Anti-CD53-Antikörper wurde für FACS, IP, IHC (fro) und IHC (p) validiert. Er ist geeignet, CD53 in Proben von Ratte zu detektieren.

Kurzübersicht für Rekombinanter CD53 Antikörper (ABIN7543047)

Target: CD53

Antikörpertyp: Recombinant Antibody

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD53 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: OX-44

Produktdetails anti-CD53 Antikörper

Verwendungszweck: Anti-CD53 [OX-44], Rabbit IgG, kappa

Spezifität: This antibody recognises CD53, which is expressed on peripheral T-cells, single (CD4 or CD8) positive thymocytes, B-cells, monocytes, macrophages, neutrophils, dendritic cells and microglial cells. This antibody recognises a conformational epitope that is dependent on disulphide bonding.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was prepared by immunizing BALB/c mice with T lymphoblasts from an MLR between purified rat T helper cells and irradiated semiallogeneic rat spleen.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody binds rat CD53, and consequently labels all myeloid cells and peripheral lymphoid cells, but only 12 % of thymocytes (Paterson, 1987). Functional studies indicate that this subset of OX-44+ thymic cells includes all thymocytes that are on the pathway to immunocompetent T lymphocytes. By contrast, the OX-44- compartment represents cells that are negative for proliferation and thymopoiesic activity, and are fated to die in situ. In addition, this antibody has been used in conjunction with CD4 and CD8 antigens to define seven subsets of thymic cell. Most CD4-CD8- cells are OX-44+, whereas almost all CD4+ CD8+ cells were OX-44-

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD53

Target: CD53

Andere Bezeichnung: CD53

Hintergrund: Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Leukocyte antigen MRC OX-44

UniProt: P24485