Rekombinanter CD86 Antikörper

Produktnummer ABIN7543021

Dieses Anti-CD86-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD86 in FACS und IF. Geeignet für Ratte.

Kurzübersicht für Rekombinanter CD86 Antikörper (ABIN7543021)

Target: CD86

Antikörpertyp: Recombinant Antibody

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD86 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: OX-48

Produktdetails anti-CD86 Antikörper

Verwendungszweck: Anti-CD86 [OX-48], Rabbit IgG, kappa

Spezifität: OX-48 binds specifically to CD86 targets which are present on subsets of T and B blasts, spleen macrophages and polymorphs, peritoneal macrophages, dendritic cells. OX48 does not appear to bind the same epitope of CD86 as 24F (Stephens et al. 2004). CD86 is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes - expression is rapidly upregulated by B cell specific stimuli. CD86 acts as a ligand for CD28 and CTLA-4. CD86 works in tandem with CD80 to prime T cells. 2 splice isoforms exist.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: OX-48 was prepared by immunizing mice with activated rat T cell blasts

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: OX-48 blocks IL-2 production and proliferation of T cells (except when in exponential phase) (Samoza et al. 1990). The antibody can also be used for FC (Stephens et al. 2004), FACS and IPs. Ox-48 has been shown to be able to block binding of CD86 with its ligand CD28.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD86

Target: CD86

Andere Bezeichnung: CD86

Hintergrund: B72, B-72, OX48, MRC OX-48, MRC OX48

UniProt: Q62810

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation