Rekombinanter Transferrin Receptor Antikörper

Produktnummer ABIN7543000

Der Maus Monoklonal anti-Transferrin Receptor Antikörper (Klon OX-26) (ABIN7543000) detektiert spezifisch Transferrin Receptor in FACS, WB, IF, IHC (fro) und in vivo. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für Rekombinanter Transferrin Receptor Antikörper (ABIN7543000)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Receptor Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), In vivo Studies (in vivo)

Klon: OX-26

Produktdetails anti-Transferrin Receptor Antikörper

Verwendungszweck: Anti-CD71 [OX-26], Mouse IgG2a, kappa

Spezifität:

This antibody is specific for the rat transferrin receptor, also known as CD71.

Application Notes original Clone: The original format of this antibody (mouse IgG2a) was developed and demonstrated to effectively labele transferrin receptor-expressing cells, including dividing lymphocytes and erythroid precursors, with minimal binding to non-dividing lymphoid cells or mature erythrocytes. It was used for fluorescence-based cell sorting to isolate subpopulations of rat hematopoietic stem and progenitor cells from bone marrow and fetal liver (Jefferies et al., 1985, PMID: 2981766). The original format of the antibody was employed in immunohistochemical staining of cryostat sections from rat brain. In vivo testing in PVG/c rats involved injecting the antibody intravenously, followed by cryostat sectioning and staining. The results showed clear labelling of rat brain capillaries, especially in the cerebellum and cortex, with weaker labelling after 16 hours. No such labelling was observed in other rat tissues, supporting the specificity for brain capillaries (Jefferies et al., 1984, PMID: 6095085). This antibody was utilized as a drug carrier to deliver compounds across the blood-brain barrier (BBB) in vivo in Sprague-Dawley rats. The antibody demonstrated selective, dose-dependent binding to brain capillary endothelial cells when administered intravenously, with localization observed as early as 5 minutes post-injection and evidence of transcytosis into the brain parenchyma over time. When conjugated with methotrexate (MTX), the antibody retained its targeting capabilities, facilitating significant accumulation of the drug in the brain parenchyma compared to free MTX (Friden et al., 1991, PMID: 2052557). This antibody was used to deliver nerve growth factor (NGF) across the BBB in in vivo experiments with aged Fischer 344 rats. The OX-26-NGF conjugate was administered intravenously and demonstrated significant therapeutic effects, including enhanced cholinergic markers in grafted tissue, improvement in spatial learning in aged rats, and upregulation of NGF receptors (Granholm et al., 1998, PMID: 9683326). Mutational screening showed that this antibody's crossing of the BBB is dependent on the its affinity to the receptor (Thom et al., 2018, PMID: 29485883). The antibody can also be used in flow cytometry, immunofluorescence, and western blot, and it may also be used in frozen-section IHC.

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with phytohaemagglutinin (PHA)-activated PVG rat lymph node cells with NS 1/1.Ag 4.1 myeloma cells.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: CD71

Hintergrund: Transferrin receptor, TfR, p90, T9, TFR1, OX26, MRC OX26, MRC OX-26

UniProt: Q99376

Pathways: Transition Metal Ion Homeostasis