SMAD1 Antikörper (Internal Region)

Produktnummer ABIN7540633

Hersteller Produkt- Nr.: 600-401-mn6s

Dieses Anti-SMAD1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SMAD1 in WB, ELISA, IHC, IF und FM. Geeignet für Maus.

Kurzübersicht für SMAD1 Antikörper (Internal Region) (ABIN7540633)

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-SMAD1 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-mn6s

Hersteller: Rockland

Verwendungszweck: Smad1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against mouse Smad1.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-Smad1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Smad1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: 1:400-1:500

IF_Microscopy_Dilution: 5-10 μg/mL

Western_Blot_Dilution: 5 μg/mL

Kommentare:

Anti-Smad1 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~52.2kDa in western blot using appropriate tissues or lysates.  Positive control used: Mouse testis and C2C12 in Western Blot, C2C12 in Immunofluorescence, Mouse Lung, Skeletal Muscle, and Testis in Immunohistochemistry.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Andere Bezeichnung: Smad1

Hintergrund: Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD 1, Smad1, Madh1, Madr1,Smad1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro, this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.

Gen-ID: 17125

NCBI Accession: NP_032565

UniProt: P70340

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development