LC3B Antikörper (Internal Region)

Produktnummer ABIN7540631

Hersteller Produkt- Nr.: 600-401-mn5s

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LC3B in WB, ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für LC3B Antikörper (Internal Region) (ABIN7540631)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LC3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-LC3B Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-mn5s

Hersteller: Rockland

Verwendungszweck: LC3B Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human LC3B.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-LC3B antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Microtubule-associated proteins 1A/1B light chain 3B conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

Flow_Cytometry_Dilution: 1:25

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-LC3B Antibody has been tested in ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry. Positive control used U251-MG Cells in Flow and Human lymph node in IHC. Testing only recommended in WB when using recombinant or overexpressing systems. Expect a band at ~14.6 kDa in western blot using appropriate lysates or tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Andere Bezeichnung: MAP1LC3B

Hintergrund: Rabbit Anti-Microtubule Associated Protein 1 Light Chain 3 Beta Antibody, Rabbit Anti-LC3B Antibody, Microtubule-Associated Proteins 1A/1B Light Chain 3B, Autophagy-Related Ubiquitin-Like Modifier LC3 B, MAP1 Light Chain 3-Like Protein 2, MAP1A/MAP1B Light Chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-Associated Protein 1 Light Chain 3 Beta, Autophagy-Related Protein LC3 B, MAP1A/1BLC3, MAP1LC3B-A, MAP1ALC3, ATG8F, LC3B,MAP1LC3B (Microtubule Associated Protein 1 Light Chain 3 Beta) is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. It is a ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). LC3B plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Anti-LC3B Antibody is useful for researchers interested in spastic paraparesis, neuroendocrine system, neuroscience research, cancer research and apoptosis research.

Gen-ID: 81631

NCBI Accession: NP_073729

UniProt: Q9GZQ8

Pathways: Autophagie