Beclin 1 Antikörper (N-Term)

Produktnummer ABIN7540603

Hersteller Produkt- Nr.: 600-401-mg4

Dieses Anti-Beclin 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Beclin 1 in WB, ELISA, IHC, IF und FM. Geeignet für Human.

Kurzübersicht für Beclin 1 Antikörper (N-Term) (ABIN7540603)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Beclin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-Beclin 1 Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 600-401-mg4

Hersteller: Rockland

Verwendungszweck: Beclin 1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Beclin-1.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-BECLIN1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human Beclin-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100-1:200

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-BECLIN-1 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~51.8kDa in western blot using appropriate lysates.  Positive control used: U251-MG in WB,  HeLa in IF, Human spleen and colon cancer tissues in IHC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Andere Bezeichnung: BECN1

Hintergrund: Rabbit Anti-Beclin-1, Beclin1 Antibody, Beclin 1 Antibody, BECN1, Coiled-Coil Myosin-Like BCL2-Interacting Protein, Beclin 1 Autophagy Related, Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein), ATG6 Autophagy Related 6 Homolog, Protein GT197, VPS30, GT197,BECLIN1 belongs to the beclin family. Beclin 1 plays a central role in autophagy, a catabolic process of degradation induced by starvation. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. May play a role in antiviral host defense.  Protects against infection by a neurovirulent strain of Sindbis virus. Localized in the Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.  Anti-Beclin-1 Antibody is useful for researchers interested in Cancer Research including Cervix Carcinoma and Breast Cancer. 

Gen-ID: 8678

NCBI Accession: NP_001300927

UniProt: Q14457

Pathways: Autophagie