ARNTL Antikörper (N-Term)

Produktnummer ABIN7540572

Hersteller Produkt- Nr.: 600-101-ml8s

Der Ziege Polyklonal Anti-ARNTL-Antikörper wurde für WB, ELISA, IF und FM validiert. Er ist geeignet, ARNTL in Proben von Human zu detektieren.

Kurzübersicht für ARNTL Antikörper (N-Term) (ABIN7540572)

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ARNTL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-ARNTL Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 600-101-ml8s

Hersteller: Rockland

Verwendungszweck: BMAL1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Bmal1.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-BMAL1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human Bmal1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000-1:50,000

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Suggested Applications: User Optimized
Anti-BMAL1 Antibody has been tested in WB and IF.  Expect a band at ~67-69 kDa in western blot using appropriate lysates.  Positive control used:  HEK293T and Mouse Brain whole cell lysates in WB, and HeLa cells in IF. 

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening.  This vial contains a relatively low volume of reagent (25 μL).  To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial.  Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial.  Use this intermediate dilution when calculating final dilutions as recommended below.  Store the vial at -20°C or below after dilution.  Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu ARNTL

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Andere Bezeichnung: ARNTL

Hintergrund: Goat Anti-Aryl hydrocarbon receptor nuclear translocator-like protein 1 Antibody, Goat Anti-Bmal1 Antibody, Arnt3, Brain and muscle ARNT-like 1,Arntl (Aryl Hydrocarbon Receptor Nuclear Translocator Like), also known as Bmal1, is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, Angelman Syndrome and altered circadian rhythm.  Anti-Bmal1 Antibody is useful for researchers interested in Neuroscience research.

Gen-ID: 406

UniProt: O00327

Pathways: Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Warburg Effekt