EIF4E Antikörper (C-Term)

Produktnummer ABIN7540529

Hersteller Produkt- Nr.: 200-901-mk9s

Dieses Huhn Polyklonal-Antikörper erkennt spezifisch EIF4E in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EIF4E Antikörper (C-Term) (ABIN7540529)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reaktivität: Human

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser EIF4E Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-EIF4E Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 200-901-mk9s

Hersteller: Rockland

Verwendungszweck: EIF4E Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human EIF4E.

Aufreinigung: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen: Anti-EIF4E antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a near C-Terminal portion of human EIF4E conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 5 μg/mL

Kommentare:

Anti-EIF4E Antibody has been tested in Western Blot and IHC.  Expect a band at ~25-28kDa in western blot using appropriate tissues and lysates.  Positive control used: HEK293T, MOLT-4 and Daudi lysates in Western Blot, Human Lung Adenocarcinoma Tissue​ in Immunohistochemistry.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Andere Bezeichnung: EIF4E

Hintergrund: Chicken Anti-EIF4E Antibody, Eukaryotic Translation Initiation Factor 4E, MRNA Cap-Binding Protein, EIF-4F 25 KDa Subunit, EIF4EL1, EIF-4E, EIF4F, Eukaryotic Translation Initiation Factor 4E-Like 1, AUTS19, EIF4E1, EIF4E, CBP,EIF4E (Eukaryotic Translation Initiation Factor 4E) recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. It is a component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. EIF4E is associated with Autism 19 and Pervasive Developmental Disorder. Anti-EIF4E Antibody is useful for researcher interested in cancer research and AKT signaling.

Gen-ID: 1977

NCBI Accession: NP_001124150

UniProt: P06730

Pathways: BCR Signaling