UCHL1 Antikörper

Produktnummer ABIN7540520

Hersteller Produkt- Nr.: 200-901-e52

Dieses Huhn Polyklonal-Antikörper erkennt spezifisch UCHL1 in WB, ELISA, IF, FM und MA. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für UCHL1 Antikörper (ABIN7540520)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reaktivität: Human

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser UCHL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Produktdetails anti-UCHL1 Antikörper

Hersteller Produkt- Nr.: 200-901-e52

Hersteller: Rockland

Verwendungszweck: Ubiquitin Hydrolase (UCHL1) Antibody

Kreuzreaktivität (Details): Anti-UCHL1 antibody is directed against human UCHL1 proteins.

Aufreinigung: The antibody was purified from chicken eggs as an IgY fraction.

Immunogen: Anti-UCHL1 Antibody was produced in chicken by repeated immunizations with recombinant full length human UCHL1 extensively purified from E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:25,000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:2500

Kommentare:

Suggested Applications: Other
Anti-Ubiquitin Hydrolase antibody is tested for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 24 kDa in size corresponding to the UCHL1 proteins in Western blots in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-UCHL1 Antikörper (ABIN7540520)

Guseva, Rizvanov, Salafutdinov, Kudryashova, Palotás, Islamov: "Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo." in: Biochemical and biophysical research communications, Vol. 451, Issue 4, pp. 503-9, (2014) (PubMed).

Details zu UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Andere Bezeichnung: UCHL1

Hintergrund: UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1,UCHL1 antibody recognizes Ubiquitin C-terminal hydrolase 1 (UCHL1) which is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. Therefore, UCHL1 antibody is ideal for investigators involved in neuronal disorders and, more generally in Neuroscience.

Gen-ID: 7345

UniProt: P09936

Pathways: Feeding Behaviour