His Tag Antikörper

Produktnummer ABIN7539828

Dieses Maus Monoklonal-Antikörper erkennt spezifisch His Tag in WB, ELISA, IP, IHC, FACS und ICC. Er zeigt eine Reaktivität gegenüber Tag.

Kurzübersicht für His Tag Antikörper (ABIN7539828)

Target: His Tag

Reaktivität: Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 6A8H8

Produktdetails anti-His Tag Antikörper

Verwendungszweck: His Tag Antibody

Aufreinigung: Purified antibody

Immunogen: Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/100 - 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Details zu His Tag

Target: His Tag

Substanzklasse: Tag

Hintergrund: The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.