His Tag Antikörper

Produktnummer ABIN7539828

Dieser Maus Monoklonal Antikörper detektiert spezifisch His Tag in WB, ELISA, IP, IHC, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Tag.

Kurzübersicht für His Tag Antikörper (ABIN7539828)

Target: His Tag

Reaktivität: Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 6A8H8

Produktdetails anti-His Tag Antikörper

Verwendungszweck: His Tag Antibody

Aufreinigung: Purified antibody

Immunogen: Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/100 - 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Details zu His Tag

Target: His Tag

Substanzklasse: Tag

Hintergrund: The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.