Human progesterone receptor. A and B forms are detected in Western blotting procedures but only the A form is detected in immunohistochemical procedures.
Produktmerkmale
This product is intended for the qualitative identification by light microscopy of Progesterone Receptor molecules in paraffin sections.
Aufreinigung
Tissue culture supernatant
Immunogen
Prokaryotic recombinant protein corresponding to the N-terminal region of the A form of the human progesterone receptor.
Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
324 mg/L
Buffer
This product is a liquid tissue culture supernatant containing 15 mM sodium azide as a preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.