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IgE Antikörper (APC)

Der Maus Monoklonal Anti-IgE-Antikörper wurde für FACS validiert. Er ist geeignet, IgE in Proben von Human zu detektieren.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/mL). (IgE Antikörper (APC))

Separation of human IgE positive CD45dim basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/mL). (IgE Antikörper (APC))

Flow cytometry multicolor surface staining of human leukocytes stained using anti-human IgE (4H10) APC antibody (concentration in sample 9 μg/mL) and anti-human CD45 (MEM-28) APC antibody (10 μL reagent / 100 μL of peripheral whole blood). (IgE Antikörper (APC))

3 Abbildungen

Produktnummer ABIN7505983

Kurzübersicht für IgE Antikörper (APC) (ABIN7505983)

Target

Alle IgE Antikörper anzeigen

IgE

Reaktivität

Human

Wirt

Maus

Klonalität

Monoklonal

Konjugat

Dieser IgE Antikörper ist konjugiert mit APC

Applikation

19
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2
2
1
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Flow Cytometry (FACS)

Klon

4H10

Discover our top product IgE Primärantikörper

Verwendungszweck

Anti-Hu IgE APC

Spezifität

The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7.325 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).

Aufreinigung

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

Purified human IgE.

Isotyp

IgG1
anti-IgE antibody
Reaktivität: Human ELISA, FACS Wirt: Maus Monoclonal 4G7-325 unconjugated

anti-IgE antibody (PE)
Reaktivität: Human FACS Wirt: Maus Monoclonal 4H10 PE

anti-IgE antibody (FITC)
Reaktivität: Human FACS Wirt: Maus Monoclonal BE5 FITC

anti-IgE antibody (Biotin)
Reaktivität: Human ELISA, FACS Wirt: Maus Monoclonal BE5 Biotin

anti-IgE antibody (PE)
Reaktivität: Human FACS Wirt: Maus Monoclonal BE5 PE

anti-IgE (AA 1-421) antibody
Reaktivität: Maus WB, IHC, ICC, IP Wirt: Kaninchen Polyclonal unconjugated

anti-IgE (AA 246-275) antibody
Reaktivität: Human WB Wirt: Kaninchen Polyclonal RB32377 unconjugated

anti-IgE antibody
Reaktivität: Maus ELISA, ELISpot Wirt: Ratte Monoclonal MT56E unconjugated

anti-IgE antibody
Reaktivität: Human ELISA, ELISpot Wirt: Maus Monoclonal 107 unconjugated

anti-IgE antibody
Reaktivität: Human ELISA, ELISpot Wirt: Maus Monoclonal 107-182-101 unconjugated

Applikationshinweise

Flow cytometry: Recommended dilution: 0.5-3 μg/mL

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

0.1 mg/mL

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Target

IgE

Hintergrund

Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

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