Rekombinanter CD3 epsilon Antikörper

Produktnummer ABIN7487698

Dieses Anti-CD3 epsilon-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD3 epsilon in FACS und IHC. Geeignet für Schwein.

Kurzübersicht für Rekombinanter CD3 epsilon Antikörper (ABIN7487698)

Target: CD3 epsilon (CD3E)

Antikörpertyp: Recombinant Antibody

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Klon: 898H2-6-15

Produktdetails anti-CD3 epsilon Antikörper

Verwendungszweck: Anti-CD3e [898H2-6-15], Rabbit IgG, kappa

Spezifität: Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically. It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 Ab (STH164). CD3, and the e chain in particular, is crucial for T cell receptor-dependent signal transduction across the lymphocyte membrane.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The initial identification of 898H2-6-15 showed that it recognised the T-cell receptor-associated glycoprotein CD3, in particular the e chain (Huang et al, 1999). More recently, the 898H2-6-15 VH and VL domains were reformatted into a scFv and conjugated to diphtheria toxin (Wang et al, 2011). This version of the antibody as an 'immunotoxin' has been shown to deplete circulating T cells in swine without observation of any clinical toxicities.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3e

Hintergrund: Leu4/T3, Cluster of Differentiation 3 epsilon chain, T cell glycoprotein CD3 epsilon chain

UniProt: Q7YRN2

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway