UPK1A Antikörper (AA 114-173)
Kurzübersicht für UPK1A Antikörper (AA 114-173) (ABIN7477611)
Target
Alle UPK1A Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Bindungsspezifität
- AA 114-173
-
Immunogen
- Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)
-
Isotyp
- IgG
-
-
-
-
Applikationshinweise
- IHC 1:100-1:200
-
Kommentare
-
Positive Control: Urinary bladder: A strong membranous and cytoplasmic Upk1a immunostaining should be seen in the urothelium (the staining can be limited to the top 1/3 or 2/3 of the urothelium).
Negative Control: Colon: Upk1a immunostaining should be absent in all cells of the colon mucosa.
-
Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Lagerung
- 4 °C,-20 °C,-80 °C
-
Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- UPK1A (Uroplakin 1a (UPK1A))
-
Andere Bezeichnung
- Uroplakin 1A
-
Hintergrund
- UP1A, UPIA, UPKA, TSPAN21,Uroplakin 1A antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- O00322
Target
-