Rekombinanter Nucleolin Antikörper

Produktnummer ABIN7477541

Der Kaninchen Monoklonal Anti-Nucleolin-Antikörper wurde für IHC validiert. Er ist geeignet, Nucleolin in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Nucleolin Antikörper (ABIN7477541)

Target: Nucleolin (NCL)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Nucleolin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-623R

Produktdetails anti-Nucleolin Antikörper

Immunogen: Recombinant full-length human NCL protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.

Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Nucleolin

Target: Nucleolin (NCL)

Andere Bezeichnung: Nucleolin

Hintergrund: NCL, Nucl, Nucleolin, Protein C23,Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization