Rekombinanter NAPSA Antikörper (AA 189-299)
Kurzübersicht für Rekombinanter NAPSA Antikörper (AA 189-299) (ABIN7477537)
Target
Alle NAPSA Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 189-299
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Immunogen
- Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Kidney: an at least moderate granular cytoplasmic staining should be seen in proximal tubule cells
Negative Control: Colon: staining should be absent in epithelial and other cells of the lamina propria.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- NAPSA (Napsin A Aspartic Peptidase (NAPSA))
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Andere Bezeichnung
- Napsin A
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Hintergrund
- ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA,Napsin A antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- O96009
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Pathways
- Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
Target
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