Ki-67 Antikörper (AA 2293-2478)

Produktnummer ABIN7477505

Der Maus Monoklonal Anti-Ki-67-Antikörper wurde für IHC validiert. Er ist geeignet, Ki-67 in Proben von Human zu detektieren.

Kurzübersicht für Ki-67 Antikörper (AA 2293-2478) (ABIN7477505)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ki-67 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-267M

Produktdetails anti-Ki-67 Antikörper

Bindungsspezifität: AA 2293-2478

Immunogen: Recombinant human Ki67 protein fragment (around aa 2293-2478) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Tonsil (moderate to strong nuclear staining in 80-90% of the germinal centre B-cells and in the vast majority of the suprabasal squamous epithelial cells).

Negative Control: Liver (nuclear staining reaction only in scattered hepatocytes (<1%), cytoplasmic staining is absent or only weak)

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Andere Bezeichnung: Ki-67

Hintergrund: KI-67, Ki67, KI-67 Antigen (KIA), MKI67, Proliferation related Ki-67 antigen,Ki-67 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process