Rekombinanter ErbB2/Her2 Antikörper (Extracellular Domain)
Kurzübersicht für Rekombinanter ErbB2/Her2 Antikörper (Extracellular Domain) (ABIN7477489)
Target
Alle ErbB2/Her2 Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- Extracellular Domain
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Immunogen
- Recombinant protein encoding the extracellular domain of human c-erbB2
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:50-1:200
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Kommentare
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Positive Control: Colon: Breast cancers with HER2 amplification should show strong positivity. Placenta: A faint staining should be seen at the surface membrane of the syncytiotrophoblast.
Negative Control: Breast: Normal epithelial cells must not show HER2 immunostaining.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
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Andere Bezeichnung
- HER2 / CD340
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Hintergrund
- P185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog,HER2 / CD340 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P04626
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Pathways
- RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Skeletal Muscle Fiber Development
Target
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