Rekombinanter Cytokeratin 7 Antikörper (AA 1-100)
Kurzübersicht für Rekombinanter Cytokeratin 7 Antikörper (AA 1-100) (ABIN7477437)
Target
Alle Cytokeratin 7 (KRT7) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 1-100
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Immunogen
- Recombinant fragment (around aa1-100) of human KRT7 protein (exact sequence is proprietary)
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Pancreas: CK7 staining is at least weak to moderate cytoplasmic intercalating ducts and strong in large pancreatic ducts.
Negative Control: Pancreas: CK7 staining should be absent in all acinar cells.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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Andere Bezeichnung
- Cytokeratin 7
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Hintergrund
- CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7,Cytokeratin 7 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P08729
Target
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