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Rekombinanter Chromogranin A Antikörper

Dieses Anti-Chromogranin A-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von Chromogranin A in IHC. Geeignet für Human.
Produktnummer ABIN7477423

Kurzübersicht für Rekombinanter Chromogranin A Antikörper (ABIN7477423)

Target

Alle Chromogranin A (CHGA) Antikörper anzeigen
Chromogranin A (CHGA)

Antikörpertyp

Recombinant Antibody

Reaktivität

  • 217
  • 80
  • 71
  • 31
  • 29
  • 15
  • 6
  • 5
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
Human

Wirt

  • 128
  • 105
  • 5
  • 1
Kaninchen

Klonalität

  • 135
  • 103
Monoklonal

Konjugat

  • 116
  • 17
  • 11
  • 10
  • 10
  • 10
  • 10
  • 10
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Chromogranin A Antikörper ist unkonjugiert

Applikation

  • 112
  • 109
  • 87
  • 84
  • 60
  • 54
  • 37
  • 23
  • 14
  • 13
  • 12
  • 11
  • 7
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC)

Klon

MSVA-380R
  • Immunogen

    Recombinant full-length human chromogranin A protein

    Isotyp

    IgG
  • Applikationshinweise

    IHC 1:100-1:200

    Kommentare

    Positive Control: Appendix: An at least weak to moderate staining must be seen in the axons and ganglion cells of the peripheral nerves while mucosal neuroendocrine cells exhibit a strong staining (neuroendocrine cells are not sufficient as positive controls because they express CGA so strongly, that even inefficient staining will detect its expression)

    Negative Control: Appendix: Epithelial and smooth muscle cells should be negative.

    Protokoll

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Lagerung

    4 °C,-20 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target

    Chromogranin A (CHGA)

    Andere Bezeichnung

    Chromogranin A

    Hintergrund

    Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II,Chromogranin A antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P10645

    Pathways

    Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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