CD45RA Antikörper
Quick Overview for CD45RA Antikörper (ABIN7477415)
Target
Alle CD45RA Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Immunogen
- Stimulated human leukocytes
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Tonsil: A strong membranous CD45RA immunostaining should be seen in the majority of lymphocytes.
Negative Control: Tonsil: CD45RA immunostaining should be absent in epithelial cells.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CD45RA
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Andere Bezeichnung
- CD45RA
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Hintergrund
- B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein,CD45RA antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P08575
Target
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