CD31 Antikörper (AA 625-738)

Produktnummer ABIN7477409

Der Maus Monoklonal Anti-CD31-Antikörper wurde für IHC validiert. Er ist geeignet, CD31 in Proben von Human zu detektieren.

Kurzübersicht für CD31 Antikörper (AA 625-738) (ABIN7477409)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD31 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-031M

Produktdetails anti-CD31 Antikörper

Bindungsspezifität: AA 625-738

Immunogen: Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Liver: At least a weak to moderate staining of the majority of the hepatic sinusoidal endothelial cells should be seen.

Negative Control: Liver: Hepatocytes should not show any CD31 staining.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: CD31

Hintergrund: EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA,CD31 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization