Rekombinanter CD5 Antikörper (AA 32-372)
Kurzübersicht für Rekombinanter CD5 Antikörper (AA 32-372) (ABIN7477395)
Target
Alle CD5 Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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    - 
                                            Bindungsspezifität
- AA 32-372
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                                            Immunogen
- Recombinant fragment (around aa32-372) corresponding to human CD5 protein (extracellular).
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                                            Isotyp
- IgG
 
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    - 
                                            Applikationshinweise
- IHC 1:100-1:200
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                                            Kommentare
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                        Positive Control: Tonsil: A strong CD5 staining of virtually all T-cells in both T-zones and in germinal centres as well as an at least weak to moderate staining of dispersed B-cells in the mantle zone should be seen. Negative Control: Tonsil: CD5 staining should be absent in germinal centre B-cells and in surface epithelial cells (A fraction of crypt epithelial cells may stain positive). 
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                                            Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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                                            Beschränkungen
- Nur für Forschungszwecke einsetzbar
 
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    - 
                                            Format
- Liquid
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                                            Lagerung
- 4 °C,-20 °C,-80 °C
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                                            Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
 
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    - CD5
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                                            Andere Bezeichnung
- CD5
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                                            Hintergrund
- CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5,CD5 antibody validated for immunohistochemistry on 76 different Normal Tissues
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                                            UniProt
- P06127
 Target
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