Rekombinanter Liver Arginase Antikörper (AA 300-400)
Kurzübersicht für Rekombinanter Liver Arginase Antikörper (AA 300-400) (ABIN7477377)
Target
Alle Liver Arginase (ARG1) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 300-400
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Immunogen
- Recombinant human ARG1 protein fragment (aa300-400) (Exact sequence is proprietary)
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Liver: A strong staining should be seen in hepatocytes. Spleen: A moderate t strong positivity should be seen in granulocytes.
Negative Control: Colon: Epithelial cells, smooth muscle and the vast majority of stroma cells should stain negative (few granulocytes may stain positive).
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
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Andere Bezeichnung
- Arginase-1
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Hintergrund
- Arginase 1, ARG1, liver-type arginase, type I arginase,Arginase-1 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P05089
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Pathways
- Cellular Response to Molecule of Bacterial Origin
Target
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