Iba1 Antikörper (AA 1-146)

Produktnummer ABIN7477371

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Iba1 in IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Iba1 Antikörper (AA 1-146) (ABIN7477371)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Iba1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-955M

Produktdetails anti-Iba1 Antikörper

Bindungsspezifität: AA 1-146

Immunogen: Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages.

Negative Control: Colon: AIF1 immunostaining should be absent in all non-inflammatory cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: AIF1

Hintergrund: Allograft inflammatory factor 1 (AIF1), balloon angioplasty responsive transcription (BART1), IBA1, Interferon responsive transcript 1, Ionized calcium-binding adapter molecule 1, IRT1, Microglia response factor (MRF1),AIF1 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P55008

Pathways: Smooth Muscle Cell Migration