PKLR Antikörper

Produktnummer ABIN7468839

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PKLR in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PKLR Antikörper (ABIN7468839)

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKLR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PKLR Antikörper

Spezifität: This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: K562 , Raw264.7 , rat liver , human PKLR-transfected 293T cells Validation: Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.62 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Andere Bezeichnung: pyruvate kinase L/R

Hintergrund:

Synonyms: pyruvate kinase L/R , PK1 , PKL , PKRL , RPK

Background: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Molekulargewicht: 62 kDa

Gen-ID: 5313

UniProt: P30613

Pathways: Ribonucleoside Biosynthetic Process