NDC80 Antikörper (pSer55)
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- Target Alle NDC80 Antikörper anzeigen
- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
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Bindungsspezifität
- pSer55
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser NDC80 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Kreuzreaktivität
- Human, Kangaroo rat (Dipodomys sp.), Maus, Ratte
- Aufreinigung
- Purified by antigen-affinity chromatography.
- Güteklasse
- KO Validated
- Immunogen
- Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human Hec1. The exact sequence is proprietary.
- Isotyp
- IgG
- Top Product
- Discover our top product NDC80 Primärantikörper
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- Applikationshinweise
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Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher. - Kommentare
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Positive Control: HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr) Validation: KO/KD, Orthogonal
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.26 mg/mL
- Buffer
- 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
- Andere Bezeichnung
- NDC80 kinetochore complex component (NDC80 Produkte)
- Synonyme
- CG9938 antikoerper, Dmel\\CG9938 antikoerper, Hec1 antikoerper, NDC80 antikoerper, dNdc80 antikoerper, dmNdc80 antikoerper, hec1 antikoerper, KNTC2 antikoerper, ndc10 antikoerper, tid3 antikoerper, 2610020P18Rik antikoerper, AV139730 antikoerper, HEC antikoerper, HEC1 antikoerper, Kntc2 antikoerper, kntc2l antikoerper, wu:fb52e02 antikoerper, zgc:111801 antikoerper, HsHec1 antikoerper, TID3 antikoerper, hsNDC80 antikoerper, kntc2 antikoerper, NDC80, kinetochore complex component antikoerper, CG9938 gene product from transcript CG9938-RA antikoerper, spindle pole body protein Ndc80 antikoerper, kinetochore protein Ndc80 antikoerper, NDC80 homolog, kinetochore complex component (S. cerevisiae) antikoerper, NDC80 kinetochore complex component antikoerper, NDC80 kinetochore complex component S homeolog antikoerper, NDC80 antikoerper, Ndc80 antikoerper, ndc80 antikoerper, ndc80.S antikoerper
- Hintergrund
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Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
Background: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]
- Molekulargewicht
- 80 kDa
- Gen-ID
- 10403
- UniProt
- O14777
- Pathways
- Maintenance of Protein Location
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