NEK2 Antikörper

Produktnummer ABIN7465044

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NEK2 in WB, IF, IHC (p) und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für NEK2 Antikörper (ABIN7465044)

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-NEK2 Antikörper

Kreuzreaktivität: Human

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein  corresponding to human NEK2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Andere Bezeichnung: NIMA related kinase 2

Hintergrund: NIMA related kinase 2 , HsPK21 , NEK2A , NLK1 , PPP1R111 , RP67,Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

Molekulargewicht: 52 kDa

Gen-ID: 4751

UniProt: P51955

Pathways: M Phase