HMGB1 Antikörper

Produktnummer ABIN7464828

Dieses Maus Monoklonal-Antikörper erkennt spezifisch HMGB1 in WB, IF, ICC, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für HMGB1 Antikörper (ABIN7464828)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HMGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Güteklasse: KO Validated

Klon: GT383

Produktdetails anti-HMGB1 Antikörper

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Neuro2A , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2 , A375 , mouse brain , rat brain , HMGB1-transfected 293T

Validation: KO/KD, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.73 mg/mL

Buffer: PBS, No Preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: high mobility group box 1

Hintergrund: High mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1,DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Molekulargewicht: 25 kDa

Gen-ID: 3146

UniProt: P09429

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome