LEF1 Antikörper (N-Term)

Produktnummer ABIN7464653

Dieses Anti-LEF1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LEF1 in WB. Geeignet für Human.

Kurzübersicht für LEF1 Antikörper (N-Term) (ABIN7464653)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LEF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-LEF1 Antikörper

Bindungsspezifität: N-Term

Spezifität: Knockdown/Knockout validation was supported by references (PMID:35801925)

Kreuzreaktivität: Human, Ratte

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human LEF1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Jurkat , Juarkat_NE

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Andere Bezeichnung: lymphoid enhancer binding factor 1

Hintergrund: Lymphoid enhancer binding factor 1 , LEF-1 , TCF10 , TCF1ALPHA , TCF7L3,LEF1 is a nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA, MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1, MIM 163905) (Waterman et al., 1991 [PubMed 2010090], van Genderen et al., 1994 [PubMed 7958926]).[supplied by OMIM]

Molekulargewicht: 44 kDa

Gen-ID: 51176

UniProt: Q9UJU2

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding