CD73 Antikörper

Produktnummer ABIN7464558

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch CD73 in WB, IF, ICC, IHC (p), Inh, Neut und PLA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD73 Antikörper (ABIN7464558)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD73 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Inhibition (Inh), Neutralization (Neut), Proximity Ligation Assay (PLA)

Produktdetails anti-CD73 Antikörper

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HepG2 , HCT116 , mouse placenta

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.28 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Andere Bezeichnung: 5'-nucleotidase ecto

Hintergrund: 5'-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT,Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase, EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Molekulargewicht: 63 kDa

Gen-ID: 4907

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process